Expression of Nitric Oxide Synthase(NOS) in Rat Bladders Subjected to Short-term Partial Outlet Obstruction / 대한비뇨기과학회지

PURPOSE: Nitric oxide synthase(NOS) is an important enzyme in the production of nitric oxide(NO). The constitutive type(cNOS) is expressed in the normal physiologic state, and the inducible type(iNOS) in expressed in the active immune state. cNOS is divided into an endothelial type (eNOS) and a neuronal type(nNOS). eNOS affects blood vessels, while nNOS affects nerve fibers. In the present study, we evaluated the expression of eNOS and nNOS in rat bladders with short-term partial outlet obstructions. We presupposed that NO is responsible for prolonged micturition problems after partial outlet obstruction. MATERIALS AND METHODS: Specific pathogen-free Sprague-Dawley rats weighing 250-300g were used for the study. Individual bladders were obtained from sham-operated control rats(n=5) and from experimental rats at 12 hours and 1, 2, 3, and 7 days after partial urethral obstruction(n=25). eNOS and nNOS were detected using immunochemical staining and analyzed with confocal microscopy and an image analyzer. RESULTS: eNOS and nNOS expression were detected in both the control group and in the group with partial outlet obstruction. The expression of eNOS showed a sharp increase at 3 days after obstruction and returned to normal at 7 days. The expression of nNOS was not significantly different between the two groups. CONCLUSIONS: In this study, we showed that eNOS increases in the rat bladder after partial outlet obstruction. This finding suggests that overproduction of NO may be the result of ischemic injury sustained during partial bladder outlet obstruction.

Patients with benign prostatic hyperplasia with pathologic prostatitis: The effect on the surgical outcome

PURPOSE: Benign prostatic hyperplasia (BPH) with prostatitis is a common clinical problem. There have been no previous reports of the effect of pathologic prostatitis on the improvement of lower urinary tract symptoms (LUTS) preceded by transurethral resection of prostate (TURP). Therefore, the purpose of this study was to determine the effect of pathologic prostatitis on improvement of LUTS after TURP. MATERIALS AND METHODS: From March 1996 to December 2006, 237 patients who received TURP were divided into two groups of with mild or severe pathologic prostatitis according to the pathological results of prostate tissue evaluation, International Prostate Symptom Score (IPSS), quality of life (QoL), maximum flow rate (Qmax) and the development of complications were recorded before and at 3 months follow up after surgery. RESULTS: No statistically significant differences were identified in the two groups with regard to Qmax, resection rate and complications (p>0.05). However, the IPSS and QoL were significantly different in comparisons between the two groups (p<0.05). CONCLUSIONS: Therefore, the results of this study show that BPH accompanied by pathologic prostatitis affects the improvement of LUTS, after TURP, and treatment of prostatitis may increase patients' satisfaction after surgery.